Inhibition and structure of Toxoplasma gondii purine nucleoside phosphorylase.

نویسندگان

  • Teraya M Donaldson
  • María B Cassera
  • Meng-Chiao Ho
  • Chenyang Zhan
  • Emilio F Merino
  • Gary B Evans
  • Peter C Tyler
  • Steven C Almo
  • Vern L Schramm
  • Kami Kim
چکیده

The intracellular pathogen Toxoplasma gondii is a purine auxotroph that relies on purine salvage for proliferation. We have optimized T. gondii purine nucleoside phosphorylase (TgPNP) stability and crystallized TgPNP with phosphate and immucillin-H, a transition-state analogue that has high affinity for the enzyme. Immucillin-H bound to TgPNP with a dissociation constant of 370 pM, the highest affinity of 11 immucillins selected to probe the catalytic site. The specificity for transition-state analogues indicated an early dissociative transition state for TgPNP. Compared to Plasmodium falciparum PNP, large substituents surrounding the 5'-hydroxyl group of inhibitors demonstrate reduced capacity for TgPNP inhibition. Catalytic discrimination against large 5' groups is consistent with the inability of TgPNP to catalyze the phosphorolysis of 5'-methylthioinosine to hypoxanthine. In contrast to mammalian PNP, the 2'-hydroxyl group is crucial for inhibitor binding in the catalytic site of TgPNP. This first crystal structure of TgPNP describes the basis for discrimination against 5'-methylthioinosine and similarly 5'-hydroxy-substituted immucillins; structural differences reflect the unique adaptations of purine salvage pathways of Apicomplexa.

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عنوان ژورنال:
  • Eukaryotic cell

دوره 13 5  شماره 

صفحات  -

تاریخ انتشار 2014